pcdna d1er addgene (Addgene inc)
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Addgene inc
pcdna d1er addgene
Pcdna D1er Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna+d1er+addgene/pCMV+R-CEPIA1er+(Plasmid+%2358216)/pm38513662-725-61-62
Average 94 stars, based on 77 article reviews
Pcdna D1er Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna+d1er+addgene/pCMV+R-CEPIA1er+(Plasmid+%2358216)/pm38513662-725-61-62
Average 94 stars, based on 77 article reviews
pcdna d1er addgene - by Bioz Stars,
2026-09
94/100 stars
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Real-time Polymerase Chain Reaction:Article Title: Uncoupling protein 2 and aldolase B impact insulin release by modulating mitochondrial function and Ca 2+ release from the ER. Article Snippet: .. Mouse: IRS-2-/-mice Kubota et al. (2000) N/A Oligonucleotides Primers for qPCR, see Table S2 This paper N/A Recombinant DNA Plasmid: Aldob Mouse tagged Clone ORIGENE Cat# MR205585 Plasmid: UCP2 Mouse Untagged Clone ORIGENE Cat# MC205697 Plasmid: Recombinant:Article Title: Uncoupling protein 2 and aldolase B impact insulin release by modulating mitochondrial function and Ca 2+ release from the ER. Article Snippet: .. Mouse: IRS-2-/-mice Kubota et al. (2000) N/A Oligonucleotides Primers for qPCR, see Table S2 This paper N/A Recombinant DNA Plasmid: Aldob Mouse tagged Clone ORIGENE Cat# MR205585 Plasmid: UCP2 Mouse Untagged Clone ORIGENE Cat# MC205697 Plasmid: Article Title: ERMA (TMEM94) is a P-type ATPase transporter for Mg 2+ uptake in the endoplasmic reticulum. Article Snippet: Article Plasmid Preparation:Article Title: Uncoupling protein 2 and aldolase B impact insulin release by modulating mitochondrial function and Ca 2+ release from the ER. Article Snippet: .. Mouse: IRS-2-/-mice Kubota et al. (2000) N/A Oligonucleotides Primers for qPCR, see Table S2 This paper N/A Recombinant DNA Plasmid: Aldob Mouse tagged Clone ORIGENE Cat# MR205585 Plasmid: UCP2 Mouse Untagged Clone ORIGENE Cat# MC205697 Plasmid: Software:Article Title: Uncoupling protein 2 and aldolase B impact insulin release by modulating mitochondrial function and Ca 2+ release from the ER. Article Snippet: .. Mouse: IRS-2-/-mice Kubota et al. (2000) N/A Oligonucleotides Primers for qPCR, see Table S2 This paper N/A Recombinant DNA Plasmid: Aldob Mouse tagged Clone ORIGENE Cat# MR205585 Plasmid: UCP2 Mouse Untagged Clone ORIGENE Cat# MC205697 Plasmid: Sequencing:Article Title: ERMA (TMEM94) is a P-type ATPase transporter for Mg 2+ uptake in the endoplasmic reticulum. Article Snippet: Article |
![(A) Representative traces of the intra-ER FRET signal using the intra-ER Ca2+ sensor <t>D1ER</t> in cells expressing RyR2. Stable HEK293 cells with inducible expression of the mouse RyR2 were transfected with D1ER. RyR2 expression was induced with 1 μg/ml tetracycline 18–24 hours after transfection. Time-lapse imaging of D1ER FRET was performed after 18–24 hours of RyR2 induction. Images were collected for 3 minutes at 0 [Ca2+], 3 minutes at 1 mM [Ca2+], 5 minutes at 2 mM [Ca2+], 5 minutes in the presence of 1 mM tetracycline to inhibit RyR2 and measure maximum ER Ca2+ capacity (FMax), and about 3 minutes in the presence of 20 mM caffeine to empty the ER and measure the residual FRET signal (FMin). (B–D) Activation (B) and termination (C) thresholds and fraction of the ER Ca2+ content released (D) during spontaneous oscillations (*P < 0.05, **P < 0.01, rank sum test [B], 2-tailed t test [C–D]). (E) ER store capacity determined as (FMax–FMin) and expressed as percentage of WT (rank sum test). (F) Percentage of cells showing oscillations in the store [Ca2+] (z test). (G) Average number of [Ca2+] oscillations per cell (**P < 0.01, rank sum test) (n = 40 [B–D], 49 [E], 87 [F], and 39 [G] WT cells; 44 [B–D], 61 [E], 104 [F] and 43 [G] P1124L cells from 4 independent D1ER transfections and inductions of RyR2 expression).](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_3635/pmc06483635/pmc06483635__jciinsight-4-126544-g150.jpg)